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目的:探索成年大鼠盆神经节(MPG)神经元的体外培养、鉴定的实用方法。方法:分别利用植块培养法、分离细胞培养法对成年大鼠MPG神经元进行体外培养,应用免疫组化以神经微丝单克隆抗体(NF-200)对MPG神经元进行鉴定。结果:通过上述两种方法均能成功培养出生长状态良好的MPG神经元,细胞呈NF-200反应阳性。结论:本实验成功建立了成年大鼠MPG神经元的体外培养方法,可作为神经科学研究的重要手段。
Objective: To explore the practical method of culture and identification of pelvic ganglion (MPG) neurons in adult rats. Methods: MPG neurons of adult rats were cultured in vitro by using cell culture method and cell culture method respectively. MPG neurons were identified by immunohistochemistry using neurofilament monoclonal antibody (NF-200). Results: MPG neurons with good growth status could be successfully cultured by the above two methods, and the cells were positive for NF-200. Conclusion: This experiment successfully established adult rat MPG neurons in vitro culture methods can be used as an important means of neuroscience research.