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为研究ＥＢ病毒与非霍奇金淋巴瘤（ＮＨＬ）的关系，采用ＰＣＲ、原位杂交、ＬＳＡＢ（ｌａｂｅｌｉｎｇｓｔｒｅｐｔａｖｉｄｉｖｉｎｂｉｏｔｉｎ）免疫组化技术，检测３２例ＮＨＬ和３３例反应性增生淋巴结中的ＥＢＶＢＮＬＦ１基因片段及其表达产物潜伏膜蛋白（ＬＭＰ１）。ＮＨＬ中ＥＢＶＢＮＬＦ１ＰＣＲ扩增阳性率为５３％，明显高于反应性增生淋巴结组２７％（Ｐ＜０．０５），其中５例Ｔ细胞淋巴瘤全部阳性。１７例ＰＣＲ阳性的ＮＨＬ中５例原位杂交阳性。ＬＭＰ１检测仅２例阳性。结果表明本组病例半数以上ＮＨＬ中有ＥＢＶ感染，可能与肿瘤的发生发展有一定关系。
To study the relationship between Epstein-Barr virus and non-Hodgkin lymphoma (NHL), PCR, in situ hybridization and LSAB (labeling streptavidivinbiotin) immunohistochemistry were used to detect EBV in 32 NHL and 33 reactive hyperplasia lymph nodes. BNLF1 gene fragment and its expression product latent membrane protein (LMP1). The positive rate of EBVBNLF1 PCR amplification in NHL was 53%, which was significantly higher than that of reactive hyperplasia lymph node group (27%) (P<0.05), of which 5 cases of T-cell lymphoma were all positive. Of the 17 NHLs with positive PCR, 5 were positive for in situ hybridization. LMP1 detected only 2 cases positive. The results showed that more than half of the patients in this group had EBV infection in the NHL, which may have a certain relationship with the occurrence and development of the tumor.