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目的研究浙江蝮蛇毒诱导人白血病K562细胞凋亡的作用及机制。方法MTT法测IC50值及细胞毒作用,Hoechest33258荧光染色法观察凋亡小体形成,流式细胞仪PI染色法检测凋亡峰和细胞周期,同时FCM及Westernblot法检测Bcl2蛋白表达。结果浙江蝮蛇毒能抑制细胞生长且呈剂量依赖关系,并能诱导人白血病K562细胞凋亡,Bcl2蛋白表达下降。结论浙江蝮蛇毒能诱导人白血病K562细胞凋亡且呈剂量依赖关系,此作用与Bcl2蛋白表达下调有关。
Objective To study the effect and mechanism of Agkistrodon acutus venom induced apoptosis in human leukemia K562 cells. Methods IC50 values and cytotoxicity were measured by MTT assay. Apoptotic bodies were observed by Hoechest 33258 staining. Apoptotic peaks and cell cycle were detected by flow cytometry and PI staining. FCM and Western blotting were used to detect the expression of Bcl2 protein. Results Agkistrodon acutus could inhibit cell growth in a dose-dependent manner and induce apoptosis in human leukemia K562 cells with decreased Bcl2 protein expression. Conclusion Agkistrodon acutus can induce the apoptosis of human leukemia K562 cells in a dose-dependent manner, which is related to the down-regulation of Bcl2 protein expression.