论文部分内容阅读
目的评估这几种检验方法在检验食品中EHEC O157:H7的特异性、敏感性。方法用平板分离培养法、酶联免疫法(ELISA)、胶体金法、荧光PCR法同时对肠出血性大肠杆菌O157:H7进行检测。结果平板分离培养法和荧光PCR法的特异性最好,高浓度时三种检测方法的敏感性接近100%,ELISA法敏感性最低86%。结论显色平板分离培养法具有最好的检测特异性和灵敏度,但检测时间较长;ELISA法灵敏度低,但快速,费用不高,无需仪器,适合基层实验室;胶体金法检测灵敏度高,有假阳性,费用高;荧光PCR法检测敏感性与特异性均较高。
Objective To evaluate the specificity and sensitivity of these test methods for the detection of EHEC O157: H7 in food. Methods Simultaneous detection of enterohemorrhagic Escherichia coli O157: H7 by plate culture, enzyme-linked immunosorbent assay (ELISA), colloidal gold method and fluorescence PCR. Results The specificity of plate culture and fluorescence PCR was the best. The sensitivity of the three detection methods was close to 100% at high concentration, and the sensitivity of ELISA was 86% at the lowest. Conclusion The method of color plate isolation and culture has the best detection specificity and sensitivity, but the detection time is longer. The ELISA method has the advantages of low sensitivity, high speed, low cost, no instrument, suitable for primary laboratory. The colloidal gold method has high sensitivity, False positive, high cost; fluorescence PCR detection of high sensitivity and specificity.