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针对韭菜生产中硝酸盐累积问题,研究了叶面喷施水杨酸(SA)对韭菜硝酸盐累积及还原同化的影响。在不同氮素水平下,叶面喷施3.0mmol·L-1SA后6～9d可明显降低韭菜叶片硝酸盐含量15.9%～21.6%,而当SA水平达到10.0mmol·L-1时会加重硝酸盐的积累。叶面喷施3.0mmol·L-1SA后9d,韭菜叶片硝酸还原酶(NR)和谷胺酰氨合成酶(GS)活性分别比对照增加了48.9%和40.3%,净光合速率和可溶性蛋白含量分别提高了43.4%和21.3%;同时,韭菜叶片谷氨酸草酰乙酸转氨酶(GOT)活性和谷氨酸丙酮酸转氨酶(GPT)活性也均有增加。因此,叶面喷施外源水杨酸显著提高了氮还原动力泵NR活性以及氮同化动力泵GS活性,同时调动光合作用和转氨作用的积极协同配合,促进了硝态氮转化为游离氨基酸和可溶性蛋白,减少了硝酸盐进入液泡贮积。
Aiming at the accumulation of nitrate in leek production, the effect of foliar application of salicylic acid (SA) on nitrate accumulation and reduction and assimilation of leeks was studied. Under different nitrogen levels, the nitrate content of leaves was significantly decreased from 15.9% to 21.6% at 6-9 days after 3.0 mmol·L-1 SA foliar spraying, while when SA level reached 10.0 mmol·L-1, Salt accumulation. Nitrogen reductase (NR) and glutamine synthetase (GS) activity in leaves of Chives increased by 48.9% and 40.3% respectively compared with the control at the 9th day after spraying 3.0mmol·L-1 SA on leaves. The net photosynthetic rate and soluble protein content Increased by 43.4% and 21.3% respectively. Meanwhile, GOT activity and glutamate pyruvate transaminase (GPT) activity in leaves of Chinese chive were also increased. Therefore, foliar application of exogenous salicylic acid significantly enhanced NR activity of nitrogen reduction power pump and GS activity of nitrogen assimilation power pump, and at the same time activated the synergistic cooperation of photosynthesis and transamination and promoted the conversion of nitrate nitrogen to free amino acid And soluble protein, reducing the nitrate into the bubble storage.