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目的探讨外周血、脾脏、胸腺趋化因子受体CCR3、CCR5、CXCR3与自然流产的关系。方法用双标记流式细胞分析技术检测自然流产模型组小鼠(CBA/J×DBA/2,n=14)、正常妊娠模型组小鼠(CBA/J×BALB/c,n=13)和正常非孕组小鼠(CBA/J,n=11)外周血、脾脏以及胸腺中CD4+T细胞CCR3、CCR5和CXCR3这三类趋化因子受体的表达。结果自然流产模型组外周血CD4+T细胞CCR3的表达率低于正常妊娠模型组(P<0.01),CCR5和CXCR3高于正常妊娠模型组(P<0.05,P<0.01);但三个指标与正常非孕组相比,差异均无统计学意义(P>0.05)。自然流产模型组脾脏CD4+T细胞CCR3的表达率低于正常妊娠模型组(P<0.01),高于正常非孕组(P<0.05);而CCR5和CXCR3高于正常妊娠模型组(P<0.05),但与正常非孕组相比,差异无统计学意义(P>0.05)。自然流产模型组胸腺CD4+T细胞CCR3的表达率低于正常妊娠模型组(P<0.05),高于正常非孕组(P<0.01);而CXCR3的表达率高于正常妊娠模型组(P<0.05),但与正常非孕组相比,差异无统计学意义(P>0.05);CCR5与其他两组相比,差异无统计学意义(P>0.05)。结论CD4+T细胞上CCR3、CCR5和CXCR3的表达异常可能在自然流产的发病中起作用。
Objective To investigate the relationship between chemokine receptors CCR3, CCR5 and CXCR3 in peripheral blood, spleen and thymus and spontaneous abortion. Methods CBA / J × DBA / 2 (n = 14), normal pregnant mice (CBA / J × BALB / c, n = 13) and normal pregnant mice were detected by dual-labeled flow cytometry The expression of CCR3, CCR5 and CXCR3 in peripheral blood, spleen and CD4 + T cells in thymus of normal non-pregnant mice (CBA / J, n = 11) Results The expression of CCR3 in peripheral blood CD4 + T cells in spontaneous abortion model group was lower than that in normal pregnancy group (P <0.01), while CCR5 and CXCR3 were higher than those in normal pregnancy group (P <0.05, P <0.01) Compared with normal non-pregnant group, the difference was not statistically significant (P> 0.05). The expression of CCR3 in spleen CD4 + T cells in spontaneous abortion model group was lower than that in normal pregnancy group (P <0.01), higher than that in normal non-pregnant group (P <0.05), while CCR5 and CXCR3 were higher than those in normal pregnancy group (P < 0.05), but no significant difference compared with normal non-pregnant group (P> 0.05). The expression of CCR3 in thymus CD4 + T cells in spontaneous abortion model group was lower than that in normal pregnancy group (P <0.05), and higher than that in normal non-pregnant group (P <0.01), while the expression rate of CXCR3 was higher than that in normal pregnancy group <0.05), but there was no significant difference compared with normal non-pregnant group (P> 0.05). There was no significant difference between CCR5 and other two groups (P> 0.05). Conclusion Abnormal expression of CCR3, CCR5 and CXCR3 on CD4 + T cells may play a role in the pathogenesis of spontaneous abortion.