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目的观察变形链球菌培养上清对EAhy926细胞的增殖活性及TLR4表达的影响。方法变形链球菌培养上清作用于EAhy926细胞,MTT法检测EAhy926细胞的增殖活性;RT-PCR法检测EAhy926细胞TLR4 mRNA的表达;流式细胞术检测EAhy926细胞表面TLR4的表达。结果不同浓度的变形链球菌培养上清作用6 h可促进EAhy926细胞的增殖(P<0.05),但12 h后可明显抑制该细胞的生长(P<0.05),呈现显著的时间和剂量依赖性。变形链球菌培养上清作用于EAhy926细胞后,TLR4 mRNA和TLR4蛋白表达量随着作用时间延长而逐渐增高,在16 h达到高峰,24 h后又逐渐下降(P<0.01)。结论变形链球菌培养上清可明显抑制EAhy926细胞的生长,上调该细胞TLR4的表达。
Objective To observe the effect of streptococcus mutans culture supernatant on the proliferation of EAhy926 cells and the expression of TLR4. Methods The supernatant of Streptococcus mutans was treated in EAhy926 cells. The proliferation of EAhy926 cells was detected by MTT assay. The expression of TLR4 mRNA in EAhy926 cells was detected by RT-PCR. The expression of TLR4 on EAhy926 cells was detected by flow cytometry. Results The supernatant of streptococcus mutans at different concentrations for 6 h promoted the proliferation of EAhy926 cells (P <0.05), but the growth of EAhy926 cells was inhibited after 12 h (P <0.05), showing a significant time and dose dependent . After treated with Streptococcus mutans, the expression of TLR4 mRNA and TLR4 protein gradually increased with the prolongation of action time, reaching the peak at 16 h and then gradually decreasing at 24 h (P <0.01). Conclusion Streptococcus mutans culture supernatant can significantly inhibit the growth of EAhy926 cells and up-regulate the expression of TLR4.