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目的:观察柿叶各提取物对链脲佐菌素(STZ)所致糖尿病小鼠的血糖、胰岛素及血脂的影响。方法:将实验小鼠随机分为13组:正常对照组,模型组,二甲双胍组,乙醇总提物高、低剂量组,氯仿萃取部位高、低剂量组,乙酸乙酯萃取部位高、低剂量组,正丁醇萃取部位高、低剂量组,水提醇沉部位高、低剂量组。除正常对照组外其它组均以腹腔注射STZ(150mg/kg)建立Ⅱ型糖尿病小鼠模型。连续给药15d后,分别测定各小鼠空腹血糖(FBG)、空腹胰岛素(FINS)和血脂。结果:与模型组比较,乙醇总提物、氯仿萃取部位、乙酸乙酯萃取部位、正丁醇萃取部位和水提醇沉部位对STZ所致糖尿病小鼠有显著降低血糖的作用,降低胰岛素抵抗指数(IR)及甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C),显著提高胰岛素敏感指数(ISI)和高密度脂蛋白胆固醇(HDL-C)。结论:柿叶提取物有降低STZ致糖尿病小鼠模型的血糖水平,改善胰岛素抵抗,调节脂代谢紊乱的作用,其作用有效成分主要存在于氯仿萃取部位、乙酸乙酯萃取部位、正丁醇萃取部位和水提醇沉部位。
Objective: To observe the effect of each extract of persimmon leaf on the blood sugar, insulin and blood lipid of diabetic mice induced by streptozotocin (STZ). Methods: The experimental mice were randomly divided into 13 groups: normal control group, model group, metformin group, ethanol total extract high and low dose group, chloroform extract high and low dose group, ethyl acetate extract high and low dose Group, n-butanol extraction site of high and low dose group, water extract and alcohol deposition site of high and low dose group. In addition to the normal control group other groups were intraperitoneal injection of STZ (150mg / kg) to establish type II diabetic mouse model. After continuous administration for 15 days, fasting blood glucose (FBG), fasting insulin (FINS) and blood lipids were determined in each mouse. Results: Compared with the model group, ethanol extract, chloroform extract, ethyl acetate extract, n-butanol extract and water extract and alcohol deposition significantly reduced blood glucose in STZ-induced diabetic mice and decreased insulin resistance IR, TG, TC and LDL-C, significantly increased ISI and HDL-C levels. Conclusion: The persimmon leaf extract can reduce the blood glucose level, improve the insulin resistance and regulate the lipid metabolism disorders in STZ-induced diabetic mice. The active ingredients are mainly in the chloroform extraction part, the ethyl acetate extraction part, the n-butanol extraction Parts and water to raise alcohol sink parts.