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目的探讨高效液相色谱-串联质谱(LC-MS/MS)法测定人血浆中氟曲马唑的效果。方法分析物和内标经乙醚和正己烷(1∶1)提取,首次采用LC-MS/MS法测定血浆中氟曲马唑浓度。色谱条件为C18色谱柱(2.1 mm×50 mm),流动相为甲醇:1‰甲酸=80∶20;多反应监测(MRM)氟曲马唑([M+H]+,m/z 279.0→183.1)和内标辛伐他汀([M+H]+,m/z 441.0→295.1)。结果在0.0996~9.96 ng/ml内,氟曲马唑与内标的峰面积比值与浓度的线性关系良好,定量限为0.0996 ng/ml,提取回收率>78.83%,日内、日间精密度良好(RSD<9.26%)。结论本次建立的LC-MS/MS法可用于测定氟曲马唑在人体血浆中的浓度。
Objective To investigate the effect of flumazole in human plasma by high performance liquid chromatography-tandem mass spectrometry (LC-MS / MS). Methods The analyte and internal standard were extracted with diethyl ether and n-hexane (1: 1), and the plasma concentration of flumexacol was determined by LC-MS / MS for the first time. Chromatographic conditions were C18 column (2.1 mm × 50 mm) with a mobile phase of methanol: 1 ‰ formic acid = 80:20; MRM flumezole ([M + H] +, m / z 279.0 → 183.1) and internal standard simvastatin ([M + H] +, m / z 441.0 → 295.1). Results In the range of 0.0996 ~ 9.96 ng / ml, the linear relationship between the peak area ratio of flumexazol and the internal standard was good and the limit of quantification was 0.0996 ng / ml. The recovery rate was> 78.83%. The intra- and inter-day precision was good RSD <9.26%). Conclusion The established LC-MS / MS method can be used to determine the concentration of flumezole in human plasma.