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本文报道了氧化/还原型谷胱甘肽对Ni~(2+)激活的钙调神经磷酸酶(Calcineurin,CaN)活性的影响。GSSG和CaN预保温导致CaN活性丧失,并且其失活过程依赖于GSSG的浓度。10mmol/L的GSSG和CaN在4℃保温,48个小时后,酶活性即全部丧失,其它实验表明,不含GSSG的酶液和含5mmol/L β—ME的酶液在4℃保温48小时后酶活性无明显变化。和GSSG保温的酶液当酶活性降至Ni~(2+)、CaM最大激活活性的20％时,透析除去GSSG,再加入GSH又能使失活的CaN活性恢复。48小时后酶活性全部丧失时GSH不能再使失活的CaN活性恢复。本文结果说明CaN是一疏基敏感酶。
This article reports the effect of oxidized / reduced glutathione on the activity of calcineurin (CaN) activated by Ni 2+. Pre-incubation of GSSG and CaN results in the loss of CaN activity, and its deactivation depends on the concentration of GSSG. 10 mmol / L GSSG and CaN incubated at 4 ℃, 48 hours after the enzyme activity that is completely lost, other experiments showed that GSSG-free enzyme solution and 5mmol / L β-ME enzyme solution incubated at 4 ℃ 48 hours After enzyme activity no significant change. When the activity of GSSG and GSSG was reduced to Ni ~ (2 +) and 20% of the maximal activity of CaM, GSSG was removed by dialysis, and the activity of inactivated CaN was restored by adding GSH. GSH can no longer restore inactivated CaN activity after 48 hours of total enzyme activity loss. Our results show that CaN is a spike-sensitive enzyme.