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杀死感染动物,从胸腔移出棉鼠丝虫,置入含0.5%葡萄糖的丝虫基础培养液(BFM)中漂洗,全虫湿重80～90mg,将其放入加或不加4.2×10~(-6)M左旋咪唑的2ml BFM液中,37℃振摇2小时(60转/分),孵育后,将虫子移至含缓冲液A(20mMNaF和8mM巯基己醇)pH 7.4的50mM双甘氨肽的匀浆器(200mg虫/ml)中研成匀浆,在2℃下10,000转离心10分钟,测定匀浆上清液内糖原磷酸化酶活力,左旋咪唑加入缓冲A液中,与全虫一起孵育,按Lowry法测蛋白含量。实验结果显示,当左旋咪唑加入未曾与药物接触的虫体匀浆时,对糖原磷酸化酶测定无影响,而在全虫制成匀浆前与药物孵育2小时,显示磷酸腺苷-独立型(磷酸化酶a)
The infected animals were killed and the fibromids were removed from the thoracic cavity and placed in a fibroin-based filamentous medium (BFM) containing 0.5% glucose for rinsing. All the animals were wetted with 80-90 mg of wet weight and placed in or without 4.2 × 10 ~ (-6) M levamisole in 2 ml of BFM and shaken at 37 ° C for 2 hours (60 rpm). After incubation, the worms were transferred to 50 mM phosphate buffer containing buffer A (20 mM NaF and 8 mM mercaptohexanol) pH 7.4 Homogenized in a homogenizer of glycylglycine (200 mg / ml), centrifuged at 10,000 rpm at 2 ° C for 10 minutes, and the glycogen phosphorylase activity in the homogenate supernatant was measured. Levamisole was added to the buffer A solution , Incubated with all insects, according to Lowry test protein content. The experimental results showed that levamisole had no effect on glycogen phosphorylase assay when homogenized with levamisole added without contact with the drug and incubated with the drug for 2 hours prior to homogenization of all the worms and showed that AMPA- Type (phosphorylase a)