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目的建立能够分泌针对肠出血性大肠杆菌O104表面抗原的特异性单克隆抗体,以期用于该病原菌的检测。方法用常规的骨髓杂交瘤融合技术。结果筛选到了2株针对肠出血性大肠杆菌O104血清型的特异性杂交瘤细胞株,凝集试验结果显示其具有良好的特异性。结论本研究筛选的单克隆抗体特异性强,敏感性高,可用于O104的检测或分离鉴定。
Objective To establish a specific monoclonal antibody that secretes the surface antigen of enterohemorrhagic Escherichia coli O104 in order to be used for the detection of this pathogen. Methods Using conventional bone marrow hybridoma fusion techniques. Results Two specific hybridoma cell lines against the enterohemorrhagic Escherichia coli O104 serotype were screened, and the results of agglutination test showed that they had good specificity. Conclusion The monoclonal antibodies screened in this study are highly specific and sensitive and can be used to detect or isolate O104.