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目的 观察腹腔感染时肺组织中肿瘤坏死因子 -α(TNF -α)和白介素 -8(IL -8)的变化 ,探讨其作用机制。方法 采用大鼠盲肠结扎并穿孔造成腹腔感染。分别在 0、2 4、48、72、96、12 0h处死一组大鼠 ,检测肺毛细血管通透性 ,取支气管肺泡灌洗液(BALF)进行细胞学分析 ,检测血浆、肺组织和BALF的TNF -α和IL -8的含量。结果 肺毛细血管通透性和BALF的中性粒细胞百分率逐渐增加 ,时间越长越明显。血浆、肺组织和BALF的TNF -α、IL -8逐渐增加 ,肺组织和BALF的TNF -α、IL -8分别显著相关 ,两者分别与血浆的TNF -α、IL -8无明显相关性。结论 TNF -α和IL -8在腹腔感染的早期阶段就参与了肺的炎症反应 ,检测BALF的TNF -α和IL -8可早期发现肠源性肺损伤
Objective To observe the changes of tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8) in lung tissue of patients with intraperitoneal infection and to explore its mechanism. Methods The cecal ligation and perforation of the rat resulted in abdominal infection. A group of rats were sacrificed at 0,2,48,72,96 and 120h, respectively. Permeability of pulmonary capillary was measured. Cytological analysis of bronchoalveolar lavage fluid (BALF) was performed to detect plasma, lung and BALF TNF-alpha and IL-8 levels. Results Pulmonary capillary permeability and BALF neutrophil percentage gradually increased, the longer the more obvious. The levels of TNF-α and IL-8 in plasma, lung tissue and BALF increased gradually. There was significant correlation between TNF-α and IL-8 in lung tissue and BALF respectively. There was no significant correlation between TNF-α and IL-8 . Conclusions TNF-α and IL-8 are involved in the inflammatory reaction in the early stages of abdominal infection. The detection of TNF-α and IL-8 in BALF may be an early detection of intestinal-derived lung injury