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目的建立耐异质性利奈唑胺葡萄球菌筛选方法,并对其耐药机制进行初步分析。方法常规方法检测葡萄球菌属临床分离株对万古霉素、替考拉宁、头孢西丁、利奈唑胺耐药性,采用菌谱分析法筛选耐异质性利奈唑胺葡萄球菌,琼脂稀释法和E-test法检测利奈唑胺MIC值,PCR方法检测其耐药cfr基因。结果未检出利奈唑胺中介或耐药菌株;耐异质性利奈唑胺金黄色葡萄球菌的检出率为1.04%;琼脂稀释法和E-test法检测异质性耐药菌株MIC结果一致;未检出利奈唑胺耐药cfr基因。结论利奈唑胺对葡萄球菌属在体外具有较强抗菌活性,该研究未检出利奈唑胺中介或耐药葡萄球菌属,其耐药机制和临床意义有待进一步研究。
Objective To establish a screening method of staphylococci resistant to linezolid and to analyze its resistance mechanism. Methods The clinical isolates of Staphylococcus were tested for their resistance to vancomycin, teicoplanin, cefoxitin and linezolid by routine methods. Staphylococcus aureus isolates were treated with bacteriological analysis, The MIC value of linezolid was detected by E-test and the cfr gene was detected by PCR. Results No linezolid or drug-resistant strains were detected. The detection rate of heterogeneity linezolid Staphylococcus aureus was 1.04%. The agar dilution method and the E-test method were consistent with the MIC results of heterogeneous drug-resistant strains Linezolid-resistant cfr gene was not detected. Conclusion Linezolid has a strong antibacterial activity against Staphylococcus aureus in vitro. The study did not detect linezolid or drug-resistant Staphylococcus aureus, its mechanism of resistance and its clinical significance need further study.