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目的研究经颅磁刺激(transcranial magnetic sti mulation, TMS)对脑梗死大鼠神经功能恢复和皮层脑源性神经营养因子(Brain derived neurotrophic factor , BDNF)表达的影响。方法成年健康雄性SD大鼠80只,随机分为脑梗死1、7、14、21、28d组及TMS1、7、14、21、28d组,每组各8只;采用线栓法制作左侧大脑中动脉闭塞的脑梗死模型;在规定的时间点行神经功能评定,应用免疫组化的方法检测梗死侧皮层BDNF的表达。结果 (1)和脑梗死28d组相比,TMS28d组大鼠Bederson神经功能评分、平衡木实验、网屏实验评分均较低,两者相比具有显著性差异(P<0.01) ;转棒实验虽评分较低,但2组无显著性差异(P>0.05) ;(2)大鼠脑梗死1d后梗死侧皮层可见较多BDNF阳性表达神经细胞,主要位于梗死灶周围,至第7d表达达高峰,然后开始下降;TMS7、14、21d组梗死侧皮层BDNF表达增加。结论 TMS能促进脑梗死大鼠神经功能恢复,机理可能与TMS能增加脑梗死侧皮层BDNF表达有关。
Objective To investigate the effects of transcranial magnetic stimulation (TMS) on the recovery of neurological function and the expression of cortical brain derived neurotrophic factor (BDNF) in rats with cerebral infarction. Methods Eighty adult male Sprague-Dawley rats were randomly divided into seven groups: 1, 7, 14, 21 and 28 days after cerebral infarction, and 7, 14, 21 and 28 days after cerebral infarction. Cerebral infarction model of middle cerebral artery occlusion was performed. Neurological function was evaluated at the prescribed time points. The expression of BDNF in infarct cortex was detected by immunohistochemistry. Results (1) Compared with 28 d cerebral infarction group, Bederson neural function score, balance beam test and screen test score of TMS28d group were lower than those of 28d cerebral infarction group (P <0.01) (P> 0.05). (2) After 1 d of cerebral infarction, more BDNF positive neurons were found in the infarct cortex, mainly around the infarct, reaching the peak on the 7th day , Then began to decline; TMS7, 14, 21d group increased cortical BDNF expression. Conclusion TMS can promote the recovery of neural function in rats with cerebral infarction. The mechanism may be related to that TMS can increase the expression of BDNF in the cortex of cerebral infarction.