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目的:研究金匮肾气丸防治SD孕鼠创伤后压力心理障碍(PTSD)所致胎损的表观遗传机制。方法:将首次受孕的36只孕鼠随机分为3组,每组12只。采用SPS法构建PTSD模型,金匮肾气丸混合饲料补肾。采用表达谱芯片检测30龄子鼠的基因表达情况,Me DIP-Seq对全基因组进行甲基化测序。结果:经表达谱芯片结果与甲基化测序结果联合分析,孕鼠模拟PTSD使得子鼠的的Ntf3基因成高甲基化状态从而抑制该基因的表达。金匮肾气丸治疗后可使得该基因的甲基化水平回复正常从而解除对该基因表达的抑制。结论:Ntf3基因的高甲基化状态可能是孕鼠PTSD胎损的表观遗传学机制,也是金匮肾气丸补肾干预起效的关键环节,为进一步研究中医调护PTSD胎损的表观遗传学机制奠定了基础。
Objective: To study the epigenetic mechanism of Jinkuishenqi pill in preventing pregnancy induced stress psychological disorder (PTSD) induced fetal loss in SD pregnant rats. Methods: Thirty-six pregnant rats who were first conceived were randomly divided into 3 groups with 12 in each group. PTSD model was established by SPS method, and Jingui Shenqi pill mixed with kidney. The gene expression profile of 30-day-old mice was detected by expression profile microarray. Me DIP-Seq was used to methylate the whole genome. Results: The results of the expression of the chip and methylation sequencing results were analyzed, pregnant mice simulated PTSD makes the offspring of Ntf3 gene hypermethylation state thereby inhibiting the expression of the gene. After treatment with Jingui Shenqi Pill, the methylation level of the gene can be returned to normal, thereby releasing the inhibition of the gene expression. CONCLUSION: The hypermethylation status of Ntf3 gene may be the epigenetic mechanism of PTSD loss in pregnant rats and also the key step in the effect of kidney-Qi intervention of Jinkuishenqi pill. In order to further study the epigenetic mechanism of traditional Chinese medicine Foundation.