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目的 探讨三种还原性巯基氨基酸对培养人脐静脉内皮细胞的损伤效应。方法 将人脐静脉内皮细胞暴露于三种还原性巯基氨基酸 2 4小时后 ,测定细胞的乳酸脱氢酶释放率、总蛋白含量、凋亡及脂质过氧化的程度。结果 1同型半胱氨酸 (Hcy)不仅促进脂质过氧化 ,而且可诱导细胞凋亡并可致细胞坏死。 2谷胱甘肽(GSH)呈还原性 ,对细胞有全面的保护作用。 3Hcy的氧化性最强 ,GSH则呈现出完全的还原性 ,半胱氨酸 (Cys)可能处于二者之间。结论 同型半胱氨酸可能是通过特异的抑制谷光甘肽过氧化物酶并减弱了细胞内的还原缓冲能力等机制 ,发挥与 Cys和 GSH不同的生物学效应 ,从而导致内皮细胞出现坏死和凋亡等损伤性的改变。
Objective To investigate the damage effect of three reducing sulfhydryl amino acids on cultured human umbilical vein endothelial cells. Methods After exposing human umbilical vein endothelial cells to three reducing sulfhydryl amino acids for 24 hours, the cell lactate dehydrogenase release rate, total protein content, apoptosis and the degree of lipid peroxidation were determined. Results Homocysteine (Hcy) not only promoted lipid peroxidation but also induced apoptosis and necrosis of cells. 2 glutathione (GSH) was reduced, the cells have a comprehensive protective effect. 3Hcy has the strongest oxidative activity, GSH shows complete reducibility, and cysteine (Cys) may be in between. Conclusion Homocysteine may exert biological effects different from those of Cys and GSH through the specific inhibition of glutathione peroxidase and the reduction of intracellular reductive buffering, leading to endothelial cell necrosis and apoptosis Death and other damage changes.