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目的采用放射受体分析方法测定鸡视网膜多巴胺受体的理化特性，研究多巴胺系统与剥夺性近视和药物治疗近视相关的机理。方法幼鸡分组：随机缝合一侧眼睑组；缝合眼睑加阿朴吗啡（ａｐｏｍｏｒｐｈｉｎｅ）结膜下注射组；正常对照组。两周后检查屈光及眼球径，分组取视网膜，用［３Ｈ］ＳＣＨ２３３９０和［３Ｈ］螺哌酮（ｓｐｉｐｅｒｏｎｅ）与各组视网膜受体蛋白进行饱和试验。结果单纯缝合眼睑形成高度近视且视网膜多巴胺两种亚型受体（Ｄ１，Ｄ２）数量增多；使用阿朴吗啡可以抑制缝合眼睑形成的近视，视网膜多巴胺受体（Ｄ１，Ｄ２）数量减少。两种受体的亲和力无显著改变。结论剥夺性近视鸡视网膜多巴胺受体均为超敏状态，与多巴胺生物水平下降有关。阿朴吗啡不同程度地影响多巴胺受体，其意义是为寻找和筛选治疗近视的药物提供可能的途径。
OBJECTIVE: To determine the physicochemical properties of retinal dopamine receptors in chicken by radioimmunoassay (RIA) and to study the mechanism of dopamine deprivation myopia and drug treatment of myopia. Methods: The young chickens were randomly divided into two groups: stitched eyelid group, suture eyelid plus apomorphine subconjunctival injection group, and normal control group. Refractive and eyeball diameters were examined two weeks later and the retina was divided into groups and saturated with [3H] SCH23390 and [3H] spiperone with each group of retinal receptor proteins. Results The number of retinal dopamine subtypes (D1 and D2) increased with the simple suture of the eyelid, and the number of retinal dopamine receptors (D1 and D2) was reduced with apomorphine. There was no significant change in the affinity of the two receptors. Conclusion The retinal dopamine receptors in deprived myopic chickens are all hypersensitive, which is related to the decrease of dopamine biological level. Apomorphine affects dopamine receptors to varying degrees, and its significance is to find possible ways to find and screen drugs for the treatment of myopia.