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基因活化剂使用浓度(A)设3个水平:375×(A1)、750×(A2)和1 500×(A3)稀释液,处理时间(B)设3个水平:浸渍苕秧基部0.5 h(B1)、1 h(B2)和2 h(B3),处理后培养在改良Hoagland培养基中,12 d后测定根系α-萘胺氧化速率、NO3-和K+吸收速率.结果表明:A1、A2和A3的α-萘胺氧化速率分别比对照(98.46μg/g.h)提高2.99,5.92和2.37倍;NO3-吸收速率分别比对照(0.23 mg/株.h)提高0.51,1.14和0.66倍;K+吸收速率分别比对照(0.12 mg/株.h)提高1.73,2.73和1.16倍.与对照相比,B1、B2和B3的α-萘胺氧化速率分别提高5.50,3.29和2.49倍;NO3-吸收速率分别提高0.60,1.09和0.63倍;K+吸收速率分别提高1.57,2.66和1.40倍.因素A、B与对照差异达极显著水平,不同水平间差异极显著(p<0.01).A2B2是最佳水平组合,即用750倍的基因活化剂浸渍苕秧基部1 h,可显著提高紫肉甘薯的根系活力,比对照α-萘胺氧化速率、NO3-和K+吸收速率分别提高5.75,1.51,3.52倍.
Gene activator concentration (A) set the three levels: 375 × (A1), 750 × (A2) and 1 500 × (A3) dilution, treatment time (B) set three levels: (B1), 1 h (B2) and 2 h (B3), respectively, and cultured in modified Hoagland medium for 12 days. The root a-naphthylamine oxidation rate, NO3- and K + The oxidation rates of α-naphthylamine in A2 and A3 were increased by 2.99, 5.92 and 2.37 times than the control (98.46 μg / gh), respectively; the NO3-uptake rates were increased by 0.51, 1.14 and 0.66 times than the control (0.23 mg / K + uptake were 1.73, 2.73 and 1.16 times higher than that of the control (0.12 mg / plant · h) respectively.Compared with the control, the oxidation rates of α-naphthylamine in B1, B2 and B3 were increased by 5.50, 3.29 and 2.49 times, The absorption rate increased by 0.60, 1.09 and 0.63 times respectively, and the K + absorption rate increased by 1.57, 2.66 and 1.40 times respectively.The differences between A, B and control reached extremely significant levels, and the differences among different levels were significant (p <0.01) Compared with the control α-naphthylamine oxidation rate, the NO3- and K + absorption rates increased by 5.75 and 1.51, respectively, 3.52 times.