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目的:探讨手霉素对U937白血病细胞的作用及其机制。方法:2μmol/L的手霉素处理U937白血病细胞不同时间,分别使用Annexin V/PI试剂及ROS检测试剂标记细胞后,应用流式细胞术检测细胞凋亡及细胞内ROS生成,免疫印迹术检测PI3K、P-Akt、Akt等蛋白的表达。结果:与0 h对照组相比,随着处理时间的延长,手霉素诱导的U937细胞凋亡(P<0.05)及ROS生成逐渐增加(P<0.05),对PI3K/Akt信号通路活化的抑制逐渐增强。N-乙酰基-L-半胱氨酸(NAC)(能有效抑制ROS产生的物质)可以阻断手霉素对U937细胞内PI3K/Akt信号通路的抑制及手霉素诱导的U937细胞凋亡。结论:手霉素通过诱导ROS产生进而抑制PI3K/Akt信号通路活化来诱导U937细胞凋亡。
Objective: To investigate the effect of vancomycin on U937 leukemia cells and its mechanism. METHODS: U937 leukemic cells were treated with 2 μmol / L of mormocin for different time. After labeling cells with Annexin V / PI reagent and ROS detection reagent respectively, apoptosis and intracellular ROS production were detected by flow cytometry. PI3K, P-Akt, Akt and other protein expression. Results: Compared with the control group at 0 h, the apoptosis of U937 cells (P <0.05) and the generation of ROS increased gradually (P <0.05) with the increase of treatment time, and the activation of PI3K / Akt signaling pathway Inhibition gradually increased. N-acetyl-L-cysteine (NAC), a substance that effectively inhibits ROS production, blocked the inhibition of the PI3K / Akt signaling pathway in U937 cells and the apoptosis of U937 cells induced by penicillin . CONCLUSION: Fucoxin induces apoptosis of U937 cells by inducing the production of ROS and thereby inhibiting the activation of PI3K / Akt signaling pathway.