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目的了解丙型肝炎肝组织和血清中丙型肝炎病毒（ＨＣＶ）ＲＮＡ的含量及其相互关系。方法采用荧光定量聚合酶链反应（ＰＣＲ）检测２４例慢性丙型肝炎患者肝组织和血清ＨＣＶＲＮＡ含量。结果肝组织中ＨＣＶＲＮＡ含量（１０８至１０１２拷贝／克）与血清病毒含量（１０５至１０９．２拷贝／毫升）呈显著正相关（Ｐ＜０．０１）。每克感染肝组织ＨＣＶＲＮＡ含量高于每毫升血清的１０２至１０４拷贝。慢性活动性肝炎肝组织和血清中ＨＣＶＲＮＡ含量显著高于慢性迁延性肝炎。动态结果显示，血清ＨＣＶＲＮＡ含量与ＡＬＴ水平呈显著正相关。结论提示慢性丙型肝炎病变程度与肝组织中ＨＣＶＲＮＡ含量有关，血清中ＨＣＶＲＮＡ含量可作为监测肝内ＨＣＶＲＮＡ复制程度的一个指标
Objective To understand the content and correlation of hepatitis C virus (HCV) RNA in hepatitis C liver tissue and serum. Methods HCV RNA levels in liver tissue and serum of 24 patients with chronic hepatitis C were detected by fluorescence quantitative polymerase chain reaction (PCR). Results There was a significant positive correlation between the levels of HCV RNA (108 to 1012 copies / g) and the contents of serum virus (105 to 109.2 copies / ml) in liver tissue (P <0.01). HCV RNA content per gram of infected liver tissue is higher than 102 to 104 copies per ml of serum. Chronic active hepatitis liver tissue and serum HCVRNA levels were significantly higher than chronic persistent hepatitis. Dynamic results showed that serum HCVRNA levels were significantly and positively correlated with ALT levels. Conclusions The severity of chronic hepatitis C is related to the level of HCVRNA in liver tissue. The serum HCVRNA level may be used as an indicator to monitor the intrahepatic HCVRNA replication