论文部分内容阅读
目的:建立肾上腺素对映异构体中S-对映体的高效毛细管电泳检测方法。方法:采用未涂层熔融石英毛细管(50μm×42 cm,有效长度32 cm),以含13 mmol·L-1DM-β-CD pH 2.5的缓冲体系(含0.10 mol·L-1磷酸和0.07 mol·L-1三乙胺)为背景电解质溶液,在20 kV分离电压下,于214 nm波长处测定S-对映体。结果:肾上腺素R-对映体和S-对映体浓度分别在0.0026～0.1036 mg·mL-1范围内线性关系良好,相关系数分别为0.9995和0.9999(n=6);定量限分别为0.3和0.4μg·mL-1(S/N≥10),检测限分别为0.1μg·mL-1(S/N≥3)。结论:本方法灵敏度高,重复性好,可用于盐酸肾上腺素注射液中S-对映体的测定及稳定性研究。
Objective: To establish a high performance capillary electrophoresis method for the determination of S-enantiomer in epinephrine enantiomers. Methods: Uncoated fused silica capillaries (50 μm × 42 cm, effective length of 32 cm) were used in a buffer system containing 0.10 mmol·L-1 phosphoric acid and 0.07 mol·L-1 phosphate buffer containing 13 mmol·L-1 DM-β- · L-1 triethylamine) as the background electrolyte solution, the S-enantiomer was measured at 214 nm at 20 kV separation voltage. Results: The linear relationship between the enantiomeric concentrations of R-enantiomer and S-enantiomer was in the range of 0.0026-0.1036 mg · mL-1, with correlation coefficients of 0.9995 and 0.9999 (n = 6) respectively. The limits of quantitation were 0.3 And 0.4μg · mL-1 (S / N≥10), the detection limits were 0.1μg · mL-1 (S / N≥3). Conclusion: This method has high sensitivity and good reproducibility. It can be used for the determination of S-enantiomer and its stability in epinephrine hydrochloride injection.