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目的观察肿瘤坏死因子-α(TNF-α)单克隆抗体(英夫利西)对矽尘诱导的大鼠肺纤维化的抑制作用,探讨英夫利西治疗肺纤维化的可能机制。方法将48只雄性Wistar大鼠随机分为3组,每组16只。染尘组和干预组大鼠气管内一次性注入50 mg SiO_2粉尘,次日起干预组给予15 mg/kg英夫利西皮下注射,共5 d,染尘组仅注射等量生理盐水;对照组气管内和皮下均注射生理盐水。各组分别于建模后7 d、14 d处死大鼠各8只,以HE、Masson染色对肺组织炎症和纤维化程度进行评价。ELISA法检测血、支气管肺泡灌洗液(BALF)中TNF-α含量,Western blot检测肺组织NF-κB蛋白表达量。结果英夫利西干预后,肺泡炎评分(7 d)和纤维化评分(14 d)优化;血清TNF-α含量降低,BALF中TNF-α含量仅7 d降低,7 d时NF-κB p65蛋白表达下调,以上结果差异均具有统计学意义(P<0.05)。结论TNF-α单克隆抗体可降低TNF-α水平,进而抑制NF-κB信号通路的活化,缓解矽尘诱导的大鼠肺纤维化进程。
Objective To investigate the inhibitory effect of TNF-α monoclonal antibody (INF-L) on silica-induced pulmonary fibrosis in rats and to explore the possible mechanism of the effect of Infliximab on pulmonary fibrosis. Methods Forty eight male Wistar rats were randomly divided into three groups of 16 rats. Dust group and intervention group rats were injected 50 mg SiO 2 into the trachea once a day, and subcutaneous injection of 15 mg / kg of Infliximab was injected subcutaneously from the next day for 5 days. The control group Intratracheal and subcutaneous injection of saline. The rats in each group were sacrificed on the 7th day and the 14th day respectively after the model was established, and the severity of inflammation and fibrosis were evaluated by HE and Masson staining. The levels of TNF-α in blood and bronchoalveolar lavage fluid (BALF) were detected by ELISA and the expression of NF-κB in lung tissue were detected by Western blot. Results The alveolitis score (7 d) and fibrosis score (14 d) were optimized after infliximab intervention. The level of TNF-α in serum was decreased and the content of TNF-α in BALF was decreased only 7 d. After 7 d, the expression of NF- The expression was down-regulated, the above differences were statistically significant (P <0.05). Conclusions TNF-α monoclonal antibody can reduce the level of TNF-α, and then inhibit the activation of NF-κB signaling pathway and relieve the process of silica-induced pulmonary fibrosis in rats.