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1958年戴维斯用大麦(4x)和球茎大麦(4x)杂交,获得完全像大麦的植株,开始他以为是两物种杂交不亲和孤雌生殖所致。后来证实,授粉后精、卵发生了受精,但在合子发育过程中,球茎大麦的染色体消失,只留下大麦的染色体,胚是单倍体的,因而培养产生了单倍体植株。这种单倍体植株是发生在生活周期的二倍期,也就是无性世代,因此是来源于体细胞的单倍体。后来加拿大学者卡加于1970年用大麦(4x)×球茎大麦(4x),1973年用大麦(2x)×球茎大麦(2x)也获得单倍体大麦。卡加的研究具有以下三个特点:①得胚率
In 1958 Davis crossed the barley (4x) with the bulbous barley (4x) to obtain a plant entirely resembling barley, beginning with what he thought was the hybrid incompatibility between the two species. Later it was confirmed that after pollination, the eggs were fertilized, but during the development of zygotes, the chromosomes of bulbous barley disappeared, leaving only the chromosomes of barley. The embryos were haploid and haploid plants were cultured. This haploid plant occurs twice as long as the life cycle, which is also asexual generation and is therefore derived from somatic haploid. Later Canadian scholar Caga used barley (4 ×) × bulblets (4 ×) in 1970 and haploid barley with barley (2x) × bulbleled barley (× 2 ×) in 1973. Cagar’s research has the following three characteristics: ① embryo rate