目的　体外表达人基质金属蛋白酶 - 2 (MMP - 2 )的催化域 ,为进一步研究其在肿瘤侵袭和转移中的作用打下基础。方法　通过PCR方法获得了编码MMP - 2催化域 (MMP - 2 -C)的cDNA序列 ,并构建了重组质粒pGEX - 5X - 3/MMP - 2-C ,由IPTG诱导在大肠杆菌中表达 ,表达产物被复性后鉴定酶活性。结果　表达产物为 6 8-Kda的融合蛋白且具有明胶酶活性。结论　体外表达的具有催化活性的融合蛋白可用于研究抗肿瘤转移药物 Objective To express the catalytic domain of human matrix metalloproteinase - 2 (MMP - 2) in vitro and lay a foundation for further study of its role in tumor invasion and metastasis. Methods The cDNA sequence encoding the catalytic domain of MMP - 2 (MMP - 2 - C) was obtained by PCR and the recombinant plasmid pGEX - 5X - 3 / MMP - 2 - C was constructed and expressed in E. coli by IPTG The product was refolded to identify enzyme activity. Results The expressed product was a 6 8-Kda fusion protein with gelatinase activity. Conclusion The in vitro expressed fusion protein with catalytic activity can be used to study anti-metastatic drugs