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采用16S rRNA基因末端限制性片段长度多态性分析(Terminal restriction fragment length polymorphism,T-RFLP)和实时荧光定量PCR(Quantitative real-time PCR,qPCR)技术,研究了玉米不同生长时期土壤古菌群落组成和丰度的变化。结果表明:1.不同生长时期,根际土和非根际土古菌优势种群无显著变化,仅在各自样品中所占比例不同,古菌群落组成无明显分离。2.乳熟期和完熟期古菌Shannon指数显著高于拔节期和抽雄期(P<0.05),根际土抽雄期古菌Evenness指数显著低于其他生长时期(P<0.05),而非根际土不同生长时期间Evenness指数差异不显著(P>0.05)。同一生长时期根际土和非根际土间Shannon指数和Evenness指数均无显著差异(P>0.05)。3.硝态氮是影响土壤古菌群落结构的关键因子。4.土壤古菌16S rRNA基因丰度随生长时期的推进呈现先升高后降低的趋势,乳熟期最高,拔节期最低,乳熟期显著高于拔节期和完熟期(P<0.05),与抽雄期差异不显著(P>0.05)。同一生长时期根际土和非根际土间无显著差异(P>0.05)。
Using 16S rRNA gene restriction fragment length polymorphism (T-RFLP) and real-time quantitative PCR (qPCR), the effects of archaeal community of archaea Changes in composition and abundance. The results showed that: 1. There was no significant change in the dominant populations of archaeal rhizosphere soil and non-rhizosphere soil at different growth stages, with only a different proportion in their respective samples and no significant separation of archaeal community composition. The Shannon index of archaeal milky and maturity were significantly higher than those at jointing and tasseling stages (P <0.05). The evenness index of archaea in the rhizosphere soil was significantly lower than that in other growth stages (P <0.05) Evenness index was not significant (P> 0.05). There was no significant difference in Shannon index and Evenness index between rhizosphere soil and non-rhizosphere soil in the same growth period (P> 0.05). Nitrate-N is the key factor affecting the archaeal community structure. The abundance of soil archaeal 16S rRNA gene increased firstly and then decreased with the development of the growth period, the highest milk-maturing stage, the lowest jointing stage, and the milky mature stage were significantly higher than those at the jointing and maturing stages (P <0.05) And tasseling difference was not significant (P> 0.05). There was no significant difference between rhizosphere soil and non-rhizosphere soil in the same growth period (P> 0.05).