急性化脓性中耳炎致病菌的检出率,在医学文献上,日本和欧美之间有显著差异。因此作者们就急性化脓性中耳炎的致病菌进行了深入探索。用灭菌棉棒分别采取鼓膜切开的中耳分泌液和自然穿孔的中耳脓性分泌物,使用包括厌氧的和增菌的9种培基进行细菌培养,并作纯培养鉴定。在212例急性化脓性中耳炎中,分离出232株细菌,其中肺炎球菌占42%,流感杆菌为24%,溶血性链球菌占12%,金黄色葡萄球为18%,其他4%。其中金黄色葡萄球菌在早期的病例中极为少见。 Acute suppurative otitis media detection rate of pathogens, in the medical literature, Japan and Europe and the United States have significant differences. Therefore, the authors explored the pathogens of acute suppurative otitis media. The tympanic membrane was used to excise the middle ear secretions and natural perforation of the middle ear purulent secretions with sterilized swabs respectively. The bacteria culture was carried out using 9 kinds of culture medium including anaerobic and enrichment bacteria, and identified by pure culture. In 212 cases of acute suppurative otitis media, 232 strains of bacteria were isolated, of which 42% were pneumococci, 24% were Influenza bacilli, 12% were Hemolytic streptococci, 18% were Staphylococcus aureus and the other 4%. Staphylococcus aureus is very rare in the early cases.