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采用剪切激活血小板的方法，利用特异性放射免疫分析显示大鼠富血小板血浆（ＰＲＰ）与富血小板组分（ＰＥＦ）分别含神经肽Ｙ（ＮＰＹ）免疫活性物质９２．５９±１０．０９μｇ／Ｌ与９７．０２±３１．１１μｇ／Ｌ，高于去血小板血浆（ＰＦＰ）（１．５２±１．３２μｇ／Ｌ），与贫血小板血浆（ＰＰＰ）（１．６６±１．６１μｇ／Ｌ）中的含量（Ｐ＜０．００１）。ＰＲＰ经剪切诱发血小板聚集后，ＰＦＰｓ（剪切后）中ＮＰＹ浓度升高到２．２５±１．３７μｇ／Ｌ（Ｐ＜０．０５），ＰＰＰ经剪切后ＮＰＹ浓度则降为１．２２±１．０７μｇ／Ｌ。结果表明大鼠血小板中含有大量ＮＰＹ，经剪切激活血小板使其聚集时，可以释放。剪切后ＰＲＰｓ与ＰＥＦｓ的ＮＰＹ浓度均升高，分别为１１２．４９±２３．０μｇ／Ｌ与１２１．９１±３１．２９μｇ／Ｌ，提示剪切促进血小板合成ＮＰＹ。
Using shear-activated platelet assay, specific radioimmunoassay showed that PRP and PEF contained neuropeptide Y (NPY) immunoreactive substance 92.59 ± 10.09 μg / L and 97.02 ± 31.11μg / L respectively, higher than that of platelet-derived plasma (PFP) (1.52 ± 1.32μg / L) and PLT (1.66 ± 1.61μg / L) (P <0.001). After shear-induced platelet aggregation of PRP, the concentration of NPY in PFPs (after shearing) increased to 2.25 ± 1.37 μg / L (P <0.05), and the concentration of NPY in PPP decreased to 1 after shearing. 22 ± 1.07 μg / L. The results showed that rat platelets contain a large number of NPY, can be released by shear-activated platelet aggregation. The NPY concentrations of PRPs and PEFs increased after sheared, which were 112.49 ± 23.0μg / L and 121.91 ± 31.29μg / L respectively, suggesting that shearing could promote the synthesis of NPY.