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最近,美国的Genentech与Genetics Institute的二组科学家同时成功地克隆了凝血因子Ⅷ∶C的DNA,并使其在哺乳类细胞株中表达产生有活性的因子Ⅷ∶C。他们根据已纯化的来源于猪或人因子Ⅷ∶C的一小片段氨基酸序列,合成出寡核苷酸探针,用此探针从基因库中找出了因子Ⅷ的基因,并已弄清楚这段基因总长为186000bp,共有长度为69～3106bp不等的26个外显子,整个基因约占X染色体的0.1%。他们分别利用人的T细胞杂交瘤株或人肝获得了cDNA克隆,并根据cDNA的序列分析推断出成熟蛋白的2332个氨基酸序列。结果显示,多肽链中有与凝血因子V结构同源的区域以及与血浆铜兰蛋白结
Recently, both Genentech and Genetics Institute scientists in the United States successfully cloned the DNA of factor VIII: C and expressed it in mammalian cell lines to produce the active factor VIII: C. Based on the purified amino acid sequence of a small fragment of porcine or human factor VIII: C, they synthesized oligonucleotide probes that were used to find the gene for factor VIII from the gene pool and to find out The total length of this gene is 186000bp, there are 26 exons in length ranging from 69 to 3106bp, and the whole gene accounts for about 0.1% of the X chromosome. They obtained cDNA clones using human T cell hybridoma strains or human liver, respectively, and deduced the 2332 amino acid sequence of the mature protein based on the cDNA sequence analysis. The results showed that the polypeptide chain with coagulation factor V structural homology region and the plasma ceruloplasmin junction