论文部分内容阅读
目的:测定山西产葛根总黄酮及3种异黄酮的含量。方法:紫外分光光度法测定葛根总黄酮的含量,检测波长250nm。高效液相色谱法测定葛根3种异黄酮的含量,色谱柱为Uvis-201(4.6mm×250mm,5μm),流动相为甲醇(A)-水(B)梯度洗脱,流速为1.0mL/min,柱温为室温;检测波长250nm。结果:葛根总黄酮在4.32-21.60μg/mL范围内线性关系良好,平均回收率为99.20%,RSD为1.57%;葛根素、大豆苷、大豆苷元的进样量分别在0.2124-4.2480μg、0.1207-2.4140μg、0.0883-1.7660μg范围内呈良好的线性关系,平均回收率分别为99.96%、99.02%、99.12%,RSD分别为1.68%、2.50%、2.19%。结论:本方法简单准确,重复性好,适用于葛根总黄酮及3种异黄酮的含量测定。
Objective: To determine the content of Pueraria lobata and three isoflavones in Shanxi. Methods: UV spectrophotometry determination of Pueraria total flavonoids, detection wavelength 250nm. The content of three kinds of isoflavones in Radix Puerariae was determined by HPLC. The column was Uvis-201 (4.6 mm × 250 mm, 5 μm) and the mobile phase was gradient elution of methanol (A) -water (B) min, the column temperature is room temperature; detection wavelength 250nm. Results: The total flavonoids of Radix Puerariae showed good linearity in the range of 4.32-21.60μg / mL with the average recovery of 99.20% and RSD of 1.57%. The injection volumes of puerarin, daidzin and daidzein were 0.2124-4.2480μg, 0.1207-2.4140μg and 0.0883-1.7660μg. The average recoveries were 99.96%, 99.02% and 99.12%, respectively. The RSDs were 1.68%, 2.50% and 2.19% respectively. Conclusion: The method is simple, accurate and reproducible. It is suitable for the determination of total flavonoids and three isoflavones in Radix Puerariae.