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目的研究高脂饮食对幼鼠肿瘤坏死因子-α(TNF-α)、抵抗素水平和胰岛素受体表达的影响及其与胰岛素抵抗指数(HOMA-IR)的相关性。方法 60只SD幼年大鼠随机分为高脂组和对照组,分别采用高脂饲料和普通饲料喂养。分别在喂养3周、6周、9周末测体质量、体长和血糖;放射免疫法测定其血清胰岛素(INS)和TNF-α水平;酶联免疫法测定其抵抗素;免疫组织化学法测定其胰岛素受体(INSR)。结果高脂组INS在3周、6周和9周分别为(16.35±1.77)mIU.L-1、(22.42±2.46)mIU.L-1和(34.25±3.32)mIU.L-1,对照组分别为(10.28±1.49)mIU.L-1、(9.76±1.23)mIU.L-1和(9.87±1.11)mIU.L-1;高脂组HOMA-IR在3周、6周和9周分别为3.57±1.03、4.94±1.86和7.81±1.01,对照组为2.19±0.13、2.12±0.22和2.20±0.19,二组比较差异均有统计学意义(Pa<0.01)。高脂组肝脏细胞INSR表达比对照组显著降低(P<0.05),但肌肉细胞INSR表达与对照组无统计学差异。高脂组TNF-α和抵抗素水平均与HOMA-IR呈正相关。结论高脂喂养可诱导幼年大鼠高胰岛素血症,HOMA-IR增加,胰岛素敏感性下降,其机制与TNF-α、抵抗素分泌增高及INSR表达降低密切相关。
Objective To investigate the effects of high-fat diet on tumor necrosis factor-α (TNF-α), resistin and insulin receptor expression in young rats and its relationship with insulin resistance index (HOMA-IR). Methods Sixty young SD rats were randomly divided into high fat diet group and control group, which were fed with high fat diet and normal diet respectively. Body mass, body length and blood glucose were measured at 3, 6 and 9 weeks of feeding respectively. Serum insulin (INS) and TNF-α levels were measured by radioimmunoassay. Resistin was detected by enzyme-linked immunosorbent assay (ELISA) Its insulin receptor (INSR). Results INS in hyperlipidemic group was (16.35 ± 1.77) mIU.L-1, (22.42 ± 2.46) mIU.L-1 and (34.25 ± 3.32) mIU.L-1 at 3 weeks, 6 weeks and 9 weeks (10.28 ± 1.49) mIU.L-1, (9.76 ± 1.23) mIU.L-1 and (9.87 ± 1.11) mIU.L-1 respectively. HOMA-IR in hyperlipidemic group was significantly higher at 3 weeks, 6 weeks and 9 Week were 3.57 ± 1.03, 4.94 ± 1.86 and 7.81 ± 1.01, respectively. The control group was 2.19 ± 0.13, 2.12 ± 0.22 and 2.20 ± 0.19, respectively. The differences between the two groups were statistically significant (P <0.01). The expression of INSR in high fat diet group was significantly lower than that in control group (P <0.05), but the expression of INSR in muscle cells was not significantly different from that in control group. The levels of TNF-α and resistin in hyperlipidemia group were positively correlated with HOMA-IR. Conclusion High fat diet can induce hyperinsulinemia in young rats. HOMA-IR increases and insulin sensitivity decreases. The mechanism is related to the increase of TNF-α, the increase of resistin and the decrease of INSR.