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为了筛选适用于转录组测序的苹果果实RNA提取方法,试验以苹果叶片、野生苹果未成熟和成熟果实、栽培种苹果成熟果实为材料,采用7种方法对4种材料进行RNA提取,并通过凝胶电泳、Agilent 2100 RNA芯片对提取的总RNA进行质量检测。结果表明,采用的7种方法中有2种方法可以成功提取4种材料的RNA,证实改良CTAB法是适合转录组测序的苹果果实RNA提取方法。
In order to screen the RNA extraction method of apple fruits suitable for transcriptome sequencing, the apple fruits, apple immature and mature fruits of mature apple and ripe apples of cultivated apple were used as material, and RNA was extracted from the four materials by seven methods Gel electrophoresis, Agilent 2100 RNA chip extraction of total RNA for quality testing. The results showed that RNA extracted from four materials could be extracted successfully by two of the seven methods, which proved that modified CTAB method is suitable for RNA extraction from apple fruit by transcriptome sequencing.