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以6个豆科牧草材料的5种不同外植体(幼根、子叶、下胚轴、茎和叶片)为试材,研究了不同生长调节剂组合下的愈伤组织诱导及体细胞胚分化情况。结果表明:基因型、外植体类型和植物生长调节剂种类及其浓度均显著影响供试材料愈伤组织的诱导与体细胞胚的分化。不同基因型以‘中苜2号’紫花苜蓿和百脉根整体表现良好,平均愈伤组织诱导率分别为86.67%和90.13%,平均体细胞胚分化率分别为36.63%和50.93%;不同外植体间愈伤组织诱导率及体细胞胚分化率差异较大,其中下胚轴是理想的受体材料,表现为出愈早,体细胞胚形成和再生能力强。供试材料愈伤组织诱导和体细胞胚分化的适宜生长调节剂组合分别为2.0 mg·L~(-1) 2,4-D+0.5 mg·L~(-1) KT和0.1 mg·L~(-1) NAA+0.5 mg·L~(-1) 6-BA。
Five different explants (young root, cotyledon, hypocotyl, stem and leaf) of 6 leguminous pasture materials were used as materials to study callus induction and somatic embryo differentiation under different combinations of growth regulators Happening. The results showed that genotypes, types of explants, plant growth regulators and concentrations all significantly affected the induction of calli and the differentiation of somatic embryos. The average genotypes of ’Zhongmu No.2’ alfalfa and Lotus japonicus performed well, with the average callus induction rates of 86.67% and 90.13%, respectively. The average somatic embryo differentiation rates were 36.63% and 50.93% The difference between callus induction rate and somatic embryo differentiation rate was significant. Hypocotyls were the ideal receptor materials, showing early emergence, strong somatic embryogenesis and regeneration. The optimal combinations of growth regulators for callus induction and somatic embryogenesis were 2.0 mg · L -1 2,4-D + 0.5 mg · L -1 KT and 0.1 mg · L -1 ~ (-1) NAA + 0.5 mg · L ~ (-1) 6-BA.