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采用花粉染色体人工加倍获得的银腺杨 (Populusalba×P .glandulosa)、毛新杨 (P .tomentosa×P .bolleana)未减数 2n花粉 ,施加一定剂量的辐射处理后杂交 ,两杂交组合共获得 16株三倍体 .其中银腺杨×毛新杨组合以1470rad处理最佳 ,三倍体得率 3 .8% ;而毛新杨×银腺杨 (16 80rad)则获得了高达 12 .9%的三倍体 .三倍体整体生长水平优势明显 ,单株生长更为突出 ,2年生实生苗的苗高和地径最高 4.9m和 5 .8cm ,分别超出群体平均值的5 8%和 176 % ;并且正反交三倍体的遗传效应差异明显 .利用未减数 2n花粉 ,经花粉辐射处理杂交选育杂种三倍体 ,将是今后白杨乃至杨属植物育种的最有效途径之一 .
Populus alba × P. glandulosa and P. somentosa × P. bolleana were pollenized with pollen chromosomes without pollination by 2n pollen and were treated with a certain dose of radiation and the hybrids were obtained in total 16 triploids.Among them, the best combination of Ginkgo biloba × Populus trichocarpa could be treated with 1470 rad, the yield of triploid was 3.8%, while that of Mao Xin Yang × Ginkgo biloba (16 80 rad) was up to 12.9% Of the triploid.The overall triploid growth has obvious advantages and the growth of single plant is more prominent.The seedling height and diameter of the two-year-old seedling are 4.9m and 5.8cm, respectively, which exceed the average of 58% and 176 %, Respectively, and there was significant difference in the genetic effects between the diploid and the reciprocal crossed diploid.Using the non-subtracted 2n pollen and the hybridized triploid hybrids by pollen irradiation would be one of the most effective ways to plant poplar and even Populus.