目的探讨人脑肿瘤转化生长因子β２（ＴＧＦβ２）基因表达与其恶性进展及增殖活性的相关性。方法采用Ｎｏｒｔｈｅｒｎ杂交、免疫组化和Ｗｅｓｔｅｒｎ杂交法检测了５０例胶质瘤、３０例脑膜瘤、３个恶性胶质瘤体外细胞系和８例正常脑组织ＴＧＦβ２的表达水平。用Ｋｉ６７标记指数（Ｋｉ６７ＬＩ）检测肿瘤增殖活性。结果正常脑组织无ＴＧＦβ２表达，而脑肿瘤均表达２８ｋｂ和／或５１ｋｂＴＧＦβ２ｍＲＮＡ片段和约２５ｋｄ及３０ｋｄ的蛋白；其在胶质瘤中的表达显著高于脑膜瘤，而低恶度胶质瘤（Ⅰ、Ⅱ）、Ⅲ、Ⅳ级胶质瘤、体外细胞系四者表达水平也有显著性差异；ＴＧＦβ２表达水平与Ｋｉ６７ＬＩ呈正相关。结论ＴＧＦβ２表达水平有随肿瘤增殖活性增高而升高的趋势，提示其可能为胶质瘤恶性进展的重要原因之一。且有可能作为基因治疗的候选基因 Objective To investigate the relationship between TGFβ2 gene expression and malignant progression and proliferative activity in human brain tumor. Methods Northern blotting, immunohistochemistry and Western blotting were used to detect the expression of TGFβ2 in 50 gliomas, 30 meningioma, 3 glioma cell lines and 8 normal brain tissues. Ki67 labeling index (Ki 67LI) detection of tumor proliferation activity. Results The expression of TGFβ2 in normal brain tissue was not found in normal brain tissue. However, the expression of 28kb and / or 51kb TGFβ2mRNA and about 25kd and 30kd protein in brain tumors were significantly higher than those in meningioma, There were also significant differences in the expression levels of tumor (Ⅰ, Ⅱ), Ⅲ, Ⅳ gliomas and cell lines in vitro. The expression level of TGFβ2 was positively correlated with Ki67LI. Conclusion The expression level of TGFβ2 increases with the increase of tumor proliferation activity, suggesting that it may be one of the important reasons for the malignant progression of glioma. And may be used as gene therapy gene candidate