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目的了解中国经性途径暴露于人类免疫缺陷病毒(HIV)而未感染者(ESN)的CD4+T淋巴细胞在体外的抗HIV活性,探讨中国ESN的抗HIV感染机制。方法采用微量全血法分离培养HIV感染者的病毒株,密度梯度离心法分离ESN外周血单个核细胞后用MACS磁分选法分选出CD4+T淋巴细胞,与HIV感染者的病毒分离株共培养,检测共培养上清的HIV-1复制动力(p24抗原)。结果ESN组CD4+T淋巴细胞对M嗜性分离毒株的复制动力显著低于健康对照组(P<0.05);ESN组CD4+T淋巴细胞对M嗜性分离毒株的复制动力显著低于T嗜性分离毒株(P<0.05);ESN组CD4+T淋巴细胞对T嗜性分离毒株及实验室毒株的感染能力与健康对照组相比差异无显著性(P>0.05)。结论中国ESN的CD4+T淋巴细胞对M嗜性病毒分离株有一定抵抗作用,可能是经性接触暴露未感者抗HIV感染的主要影响因素。
Objective To investigate the anti-HIV activity of CD4 + T lymphocytes exposed to human immunodeficiency virus (HIV) and uninfected (ESN) in China through sexual route and explore the mechanism of anti-HIV infection of ESN in China. Methods The virus strains isolated from HIV-infected patients were isolated and cultured by micro-whole blood method. The peripheral blood mononuclear cells of ESN were separated by density gradient centrifugation, and the CD4 + T lymphocytes were separated by MACS magnetic separation. Co-cultures were tested for co-culture supernatant HIV-1 replication motility (p24 antigen). Results The replication of CD4 + T lymphocytes in ESN group was significantly lower than that in healthy control group (P <0.05). The replication of CD4 + T lymphocytes in ESN group was significantly lower than that of M tropic isolate T (P <0.05). The infection ability of CD4 + T lymphocytes in TN isolates and laboratory strains in ESN group was not significantly different from that in healthy controls (P> 0.05). Conclusions CD4 + T lymphocytes of Chinese ESN have some resistance to M-tropic virus isolates, which may be the main influencing factors of anti-HIV infection through non-sensitized exposure to sexual contact.