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褐飞虱是危害水稻生产最重要的害虫之一,利用寄主抗性被认为足防治褐飞虱最经济而有效的方法。斯里兰卡水稻品种Kaharamana对东亚和东南亚的褐飞虱种群均表现抗虫性,利用分子遗传学的方法对其携带的Rph9基因进行了SSR 定位。所用的遗传群体为来源于Kaharamana和02428的含有180个单株的F2分离群体,每个F2单株套袋自交获得F2:3家系。利用苗期集团鉴定对F2:3家系进行抗褐飞虱鉴定,以推测相应F2单株的基因型。连锁分析表明,Bph9位于第12染色体上的两个SSR标记RM463和RM5341之间,分别与之相距6．8 cM和9．7 cM。该标记有助于将Bph9用于分子标记辅助选择育种研究。
Nilaparvata lugens (Nilaparvata lugens) is one of the most important pests that endanger rice production. The use of host resistance is considered as the most economical and effective way to control Nilaparvata lugens. Kaharamana, a Sri Lanka rice cultivar, exhibited insect resistance to the brown planthopper population in East and Southeast Asia. The Rph9 gene carried by SSRI was mapped by molecular genetic method. The genetic population used was a F2 segregation population containing 180 individuals derived from Kaharamana and 02428, F2: 3 lines were selfing per bag of F2 plants. The seedling group identification was used to identify the F2: 3 lines against BPH to infer the genotypes of the corresponding F2 plants. Linkage analysis showed that Bph9 was located between two SSR markers RM463 and RM5341 on chromosome 12 with a distance of 6.8 cM and 9.7 cM, respectively. This marker facilitates the use of Bph9 for molecular marker-assisted selection breeding research.