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目的探讨拓扑异构酶Ⅱ(TopoⅡ)、环氧化物酶(COX)-2、血管内皮生长因子(VEGF)在三阴性乳腺癌(TNBC)患者中的表达及临床意义。方法选取住院接受治疗的乳腺癌患者112例(TNBC 24例,非TNBC88例),构建组织芯片,采用免疫组化法检测COX-2、VEGF、TopoⅡ表达情况进行检测,分析表达情况与临床病理特征之间的相关性。结果 TNBC与非TNBC患者COX-2、VEGF阳性表达率差异无统计学意义(P>0.05)。TNBC患者TopoⅡ表达阳性率为91.67%,明显高于非TNBC患者的69.32%(P<0.05)。TNBC患者TopoⅡ、VEGF、COX-2阳性表达率分别为91.67%、87.50%、75.00%,3项指标表达与患者年龄、病理学类型、肿瘤大小、脉管侵犯、组织分级无明显相关性(P>0.05),VEGF表达与淋巴结转移情况相关(P<0.05),而TopoⅡ及COX-2表达与淋巴结转移无关(P>0.05)。TNBC患者中COX-2与TopoⅡ表达、COX-2与VEGF表达、VEGF与TopoⅡ表达均有明显相关性(P<0.05)。结论 TNBC患者TopoⅡ阳性表达率较高,VEGF表达情况与淋巴结转移具有明显相关性,TopoⅡ、COX-2、VEGF 3项指标表达情况密切相关,可能共同参与了TNBC的发生发展过程。
Objective To investigate the expression and clinical significance of topoisomerase Ⅱ (Topo Ⅱ), cyclooxygenase (COX) -2 and vascular endothelial growth factor (VEGF) in triple negative breast cancer (TNBC) patients. Methods Totally 112 breast cancer patients (24 TNBC and 88 non-TNBC) were enrolled in the study. The tissue microarray was constructed and the expressions of COX-2, VEGF and TopoⅡ were detected by immunohistochemistry. The expression and clinicopathological features were analyzed The correlation between. Results The positive rates of COX-2 and VEGF in TNBC and non-TNBC patients were not significantly different (P> 0.05). The positive rate of Topo Ⅱ in TNBC patients was 91.67%, which was significantly higher than that in non-TNBC patients (69.32%, P <0.05). The positive expression rates of TopoⅡ, VEGF and COX-2 in TNBC patients were 91.67%, 87.50% and 75.00% respectively. There was no significant correlation between the three indicators and age, pathological type, tumor size, vascular invasion and histological grade > 0.05). The expression of VEGF was correlated with lymph node metastasis (P <0.05), while the expression of Topo Ⅱ and COX-2 had no correlation with lymph node metastasis (P> 0.05). The expression of COX-2 and TopoⅡ in TNBC patients was significantly correlated with the expression of COX-2 and VEGF, TopoⅡ (P <0.05). Conclusion The positive rate of Topo Ⅱ is high in TNBC patients. There is a significant correlation between the expression of VEGF and the lymph node metastasis. The expression of Topo Ⅱ, COX-2 and VEGF are closely related and may participate in the occurrence and development of TNBC.