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目的 探讨维生素E对肝脏星状细胞 (HSC)Ⅰ型胶原、转化生长因子 β1(TGFβ1)和基质金属蛋白酶组织抑制剂 2 (TIMP2 )表达的影响。方法 体外培养的HSC ,经 1mg/L维生素E作用后 ,用免疫组织化学法检测Ⅰ型胶原表达 ;原位杂交技术检测TGFβ1和TIMP2 mRNA表达。结果 实验组中维生素E能使HSCⅠ型胶原表达明显少于对照组 (P <0 .0 1) ,但对TGFβ1及TIMP2 mRNA表达无影响。结论 维生素E抗肝纤维化作用可以通过抑制Ⅰ型胶原表达实现。
Objective To investigate the effect of vitamin E on the expression of type Ⅰ collagen, transforming growth factor β1 (TGFβ1) and tissue inhibitor of matrix metalloproteinase 2 (TIMP2) in hepatic stellate cells (HSC). Methods HSC cultured in vitro was treated with 1 mg / L vitamin E, and the expression of type Ⅰ collagen was detected by immunohistochemistry. The expressions of TGFβ1 and TIMP2 mRNA were detected by in situ hybridization. Results Vitamin E in the experimental group was significantly less than that in the control group (P <0.01), but had no effect on the expression of TGFβ1 and TIMP2 mRNA. Conclusion The anti-hepatic fibrosis effect of vitamin E can be achieved by inhibiting the expression of type Ⅰ collagen.