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目的：探讨草苁蓉（ＢＲ）对大鼠肝脏化学致癌作用的抑制效应。方法：按ＳｏｌｔＦａｒｂｅｒ氏方法复制大鼠肝脏癌前病变模型，用免疫组织化学方法观察了在肝脏癌前病变组和ＢＲ处理组及正常对照组大鼠肝脏中胎盘型谷胱甘肽Ｓ转移酶（ＧＳＴＰ），突变型Ｐ５３蛋白及Ｐ２１蛋白的表达水平。结果：发现５００ｍｇ／ｋｇ剂量的ＢＲ甲醇提取物对二乙基亚硝胺（ＤＥＮ）诱发Ｆ３４４大鼠肝脏ＧＳＴＰ阳性灶的形成有抑制作用，而且其突变型Ｐ５３蛋白及ｒａｓ基因产物ｐ２１蛋白的表达水平显著低于癌前病变组，并在ＢＲ处理后，在肝脏化学致癌初期由于癌前病变的形成所增高的γＧＴ活力明显下降。结论：提示ＢＲ对ＤＥＮ诱发大鼠肝脏癌前病变有抑制作用。
OBJECTIVE: To investigate the inhibitory effect of Herba Chuanxiong (BR) on chemical liver cancer in rats. METHODS: Rat hepatic precancerous lesion models were replicated according to Solt-Farber’s method. Placental glutathione S in the liver of rats with precancerous liver lesions, BR treatment and normal controls was observed by immunohistochemistry. Transferase (GST-P), mutant P53 protein and P21 protein expression levels. RESULTS: It was found that 500mg/kg dose of BR methanol extract inhibited the formation of GEST-P positive foci in F344 rat liver induced by diethylnitrosamine (DEN), and its mutant P53 protein and ras gene product p21 protein The expression level was significantly lower than that in the precancerous lesion group. After BR treatment, the γ-GT activity increased significantly in the early stage of liver chemical carcinogenesis due to the formation of precancerous lesions. Conclusion: It suggests that BR has inhibitory effect on DEN-induced liver precancerous lesions in rats.