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目的了解长春地区2008年婴幼儿轮状病毒(Rotavirus,RV)腹泻临床及病原情况。方法用聚丙烯凝胶电泳和/或酶联免疫吸附试验及逆转录-聚合酶链反应,对2008年长春市儿童医院因腹泻住院婴幼儿粪便标本441份进行检测,并对临床资料进行分析。结果婴幼儿腹泻RV检出率53.29%;人(Human)RV(HRV)感染有3月和11月两个高峰。基因型分布:G分型,流行优势型为G(340.85%),其次为G(112.34%),然后是G2、G(96.81%),G4、G8、G11、G13、G14、G15、G1(80.43%),未分型27.66%;P分型,流行优势型为P(845.11%),P(417.02%),P(61.70%),P1(01.28%),P9、P1(10.43%),未分型34.04%;HRV感染平均月龄为(11.07±6.73),97.36%发生于≤24月龄儿童;HRV感染男、女性别比为1.87:1;临床表现发热占43.83%(103/235),呕吐占50.64%(119/235),神经系统症状占1.28%(3/235),呼吸道症状占42.13%(99/235),平均住院(5.13±2.09)d。结论 HRV是2008年长春地区婴幼儿病毒性腹泻的主要病原;HRV流行株呈现超常多样性;HRV腹泻常伴有呼吸道症状。
Objective To understand the clinical and pathogenic status of Rotavirus (RV) diarrhea in Changchun in 2008. Methods Using polypropylene gel electrophoresis, enzyme-linked immunosorbent assay and reverse transcriptase-polymerase chain reaction, 441 infant and / or stool samples from hospitalized children with diarrhea in Changchun Children’s Hospital in 2008 were tested and the clinical data were analyzed. Results The detection rate of RV in infants and young children was 53.29%. There were two peaks in March and November in Human RV (HRV) infection. Genotype distribution was G genotype with prevalence of G (340.85%) followed by G (112.34%) followed by G2, G (96.81%), G4, G8, G11, G13, G14, G15, 80.43%), and 27.66% were not typed. The prevalence of P type was P (845.11%), P (417.02%), P (61.70%), P1 The average monthly age of HRV infection was (11.07 ± 6.73), 97.36% occurred in children ≤ 24 months. The male / female ratio of HRV infection was 1.87: 1. The clinical manifestations of fever accounted for 43.83% (103/235) ), Vomiting accounted for 50.64% (119/235), neurological symptoms accounted for 1.28% (3/235), respiratory symptoms accounted for 42.13% (99/235), and average hospitalization (5.13 ± 2.09) d. Conclusion HRV is the main pathogen of infantile viral diarrhea in Changchun in 2008. The epidemic strains of HRV are extremely diverse. The HRV diarrhea is often accompanied by respiratory symptoms.