论文部分内容阅读
目的 探讨大肠癌患者外周血CK 2 0mRNA(细胞角蛋白 2 0mRNA)基因表达及其临床意义。方法 用RT PCR(逆转录 多聚酶链反应 )法检测了 34例大肠癌患者外周血CK 2 0mRNA的表达情况。结果 按Dukes分期外周血CK 2 0mRNA的表达率分别是 :A期 2 0 % ,B期 37 5 % ,C期 5 3 3 % ,D期 83 3 %。结论 大肠癌患者外周血CK 2 0mRNA的基因表达与Dukes分期有一定关系 ,并且可以作为大肠癌血行转移的分子指标之一
Objective To investigate the expression of cytokeratin 20 mRNA in peripheral blood of patients with colorectal cancer and its clinical significance. Methods RT-PCR (reverse transcription polymerase chain reaction) was used to detect the expression of CK20 mRNA in peripheral blood of 34 patients with colorectal cancer. Results The expression rate of CK 2 0 mRNA in peripheral blood by Dukes was: 20% in A stage, 37.5% in B stage, 533% in C stage and 83 3% in D stage. Conclusion The expression of CK20 mRNA in peripheral blood of patients with colorectal cancer is related to Dukes stage, and it can be used as one of the molecular indicators of blood metastasis in colorectal cancer.