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本研究利用持续光照大鼠模型,观察日节律紊乱对大鼠妊娠及其子代早期的生长发育的影响,并初步探讨其与褪黑素及孕酮变化的关系。妊娠期给予24 h持续光照,观察日节律紊乱对大鼠分娩的影响。大鼠受孕后,在不同妊娠阶段采集血清,检测褪黑素和孕酮水平。在妊娠15日龄,随机选取一半孕鼠,剖腹观察胚胎吸收率。剩余母鼠继续饲养,观察分娩情况及产后仔鼠生长发育情况。结果显示:(1)持续光照条件下的日节律紊乱,可以导致大鼠不良妊娠结局,胚胎吸收率上升,活产仔鼠数量下降;(2)持续光照条件下的日节律紊乱,可以抑制褪黑素分泌,降低孕酮水平;(3)持续光照条件下的日节律紊乱,可以干扰大鼠的分娩节律,导致分娩时程延长,但对妊娠天数没有产生影响;(4)孕期大鼠暴露于持续光照条件下的日节律紊乱状态,可以影响仔鼠的宫内发育及子代早期生长发育。以上结果表明,持续光照导致的日节律紊乱,对于妊娠大鼠及新生大鼠,均会产生不良影响。本研究为临床指导孕期女性生物节律调节以及褪黑素的应用,提供理论依据。
In this study, continuous light rat model was used to observe the effect of daily rhythm disturbance on pregnancy and its progeny growth and development in rats and to explore its relationship with changes of melatonin and progesterone. 24 hours of gestation given continuous light to observe the effects of rhythm disorders on childbirth in rats. After conception, rats were collected at different stages of pregnancy to detect the levels of melatonin and progesterone. At 15 days of gestation, half of the pregnant rats were randomly selected and observed for embryo absorption rate. The rest of the remaining mice continue feeding, observation of childbirth and postpartum offspring growth and development. The results showed that: (1) The disturbance of daytime rhythm under continuous light condition can lead to adverse pregnancy outcomes and the increase of embryo absorptivity, and the number of live offspring decreased; (2) The daily rhythm disturbance under continuous light could inhibit fading Melatonin, reduce progesterone levels; (3) continuous rhythm of the day under the conditions of rhythm disorders can interfere with the rhythm of delivery in rats, resulting in prolonged childbirth schedule, but did not affect the number of days of pregnancy; (4) pregnant rats exposed In the continuous light conditions of the rhythm disorders, can affect the pups intrauterine development and progeny early growth and development. The above results show that the continuous light caused by the rhythm disorder, both in pregnant rats and neonatal rats, will have a negative impact. This study provides a theoretical basis for the clinical guidance of biological rhythm regulation of pregnant women and the application of melatonin.