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[目的]探讨果蝇zeste基因增强子同源物2(EZH2)和脆性组氨酸三联体(FHIT)蛋白在原发性肝细胞性肝癌(HCC)组织中的表达,及其在肝癌发生发展过程中的作用和临床病理学意义。[方法]应用免疫组化S-P法检测50例原发性肝细胞性肝癌、30例肝硬化、10例正常肝组织中EZH2、FHIT蛋白的表达。[结果]FHIT蛋白在HCC中的表达明显低于肝硬化和正常肝组织,阳性表达率分别是40.00%(20/50)、80.00%(24/30)、80.00%(8/10),三者之间具有明显的差异性(χ2=15.74,P<0.05);HCC、肝硬化和正常肝组织中EZH2蛋白阳性表达率分别是88.00%(44/50)、46.67%(14/30)、30.00%(3/10),三者之间的差异有统计学意义(χ2=20.63,P<0.05),且EZH2蛋白在HCC组织中的表达明显高于肝硬化和正常肝组织中的表达(χ2=16.07,χ2=14.07,P均<0.05)。[结论]EZH2蛋白在肝癌中的表达增加与FHIT蛋白相关,提示肝癌中EZH2蛋白与FHIT蛋白可能相互协调,促进肝癌的发生发展。12
[Objective] To investigate the expression of zebrafish zeste gene enhancer homolog 2 (EZH2) and fragile histidine triad (FHIT) in primary hepatocellular carcinoma (HCC) and its role in the development of hepatocellular carcinoma The role of the process and clinical pathological significance. [Method] The expression of EZH2 and FHIT protein in 50 cases of primary hepatocellular carcinoma, 30 cases of cirrhosis and 10 cases of normal liver tissue were detected by immunohistochemical S-P method. [Results] The expression of FHIT protein in HCC was significantly lower than that in cirrhosis and normal liver tissues (40.00%, 20/50, 80.00%, 80.00%, 8/10 respectively) (Χ2 = 15.74, P <0.05). The positive rates of EZH2 protein expression in HCC, cirrhosis and normal liver tissues were 88.00% (44/50) and 46.67% (14/30), respectively 30.00% (3/10) respectively. There was significant difference among them (χ2 = 20.63, P <0.05), and the expression of EZH2 protein in HCC tissues was significantly higher than that in cirrhosis and normal liver tissues χ2 = 16.07, χ2 = 14.07, P <0.05). [Conclusion] The increased expression of EZH2 protein in HCC correlates with FHIT protein, suggesting that EZH2 protein may be coordinated with FHIT protein in hepatocellular carcinoma to promote the development of HCC. 12