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研究了不同激素组合处理对鲁梅克斯K 1杂交酸模子叶愈伤组织的诱导 ,试验结果表明 ,2 ,4 D配合使用 6 BAP时愈伤组织诱导率最高。在附加 1 0mg/L 2 ,4 D的液体MS培养基中通过悬浮培养分离出的单细胞 ,经数次继代后获得良好的悬浮培养体系
The effects of different hormones on leaf callus induction of Rumex K 1 hybrid model were studied. The results showed that callus induction rate was the highest when 2,4 D and 2 D were combined with 6 BAP. Single cells isolated by suspension culture in liquid MS medium supplemented with 10 mg / L 2, 4 D were subcultured several times to obtain a good suspension culture system