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目的半乳糖胺-过渡金属配合物的合成及其水解酶活性的研究。方法以半乳糖为原料,经三(2-氨乙基)胺连接修饰后再分别与过渡金属Cu(Ⅱ)、Ni(Ⅱ)反应合成2个配合物。以对硝基苯酚吡啶甲酸酯(PNPP)为水解模拟酶模型,研究了在2个不同pH值下所合成糖胺-过渡金属配合物的水解活性。结果通过元素分析、金属含量测定、光谱分析及摩尔电导等表征手段,确定了2个配合物化学结构为[C_(24)H_(48)N_4O_(15)CuCl]Cl·3H_2O]和[C_(24)H_(48)N_4O_(15)Ni·2H_2O]Cl_2·H_2O],而且2个配合物的水解速率远大于自发水解的速率。结论三角双锥构型的Cu(II)配合物和具有八面体构型的Ni(II)配合物都具有较好的金属水解酶活性。
Aim To study the synthesis of galactosamine - transition metal complex and its hydrolase activity. Methods Galactose was used as a starting material to prepare two complexes by the reaction of tris (2-aminoethyl) amine with Cu (Ⅱ) and Ni (Ⅱ) respectively. The p-nitrophenol picolinate (PNPP) was used as a hydrolysis model to study the hydrolytic activity of the synthesized glycosaminoglycans - transition metal complexes at two different pH values. Results The chemical structures of the two complexes were confirmed as [C_ (24) H_ (48) N_4O_ (15) CuCl] Cl · 3H_2O] and [C_ 24) H_ (48) N_4O_ (15) Ni · 2H_2O] Cl_2 · H_2O], and the hydrolysis rates of the two complexes were much higher than those of spontaneous hydrolysis. CONCLUSION Trivalent bipyramidal Cu (II) complex and Ni (II) complex with octahedral structure all have good metallohydrolase activity.