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本研究利用同源克隆法以苹果属平邑甜茶和杂种后代叶片为试验材料,克隆得到4个SERK基因家族片段,并分析了SERKs家族基因在平邑甜茶和杂种后代不同器官和组织中的表达情况。研究结果表明:分离获得的4个SERK基因家族片段的氨基酸序列与模式植物拟南芥、烟草等植物的同源性均在84%以上,同时与其他物种的氨基酸序列进行Blast比对都具有较高的同源性;RT-PCR定量分析结果表明SERK1、SERK2、SERK3、SERK4基因主要在生殖器官中表达。因此,推测SERKs基因在平邑甜茶和杂种后代生殖发育过程中起着重要的调控作用。本研究结果为进一步揭示无融合生殖的发生机理奠定了基础。
In this study, four homologous cloning methods were used to clone four SERK gene families from the leaves of Malus hupehensis and hybrid progenies, and the expression of SERKs family genes in different organs and tissues of Malus hupehensis and hybrid progenies Happening. The results showed that the amino acid sequences of the four segregated SERK gene families were more than 84% homologous to that of the Arabidopsis thaliana and tobacco plants, and the Blast comparison with the amino acid sequences of other species also showed significant differences High homology; RT-PCR quantitative analysis results show that SERK1, SERK2, SERK3, SERK4 gene mainly in the reproductive organs. Therefore, it is speculated that SERKs plays an important regulatory role in reproductive development of Malus hupehensis and hybrid offspring. The results of this study laid the foundation for further revealing the mechanism of apomixis.