论文部分内容阅读
目的研究白藜芦醇苷(PD)对内毒素休克大鼠肺组织中Clara细胞分泌蛋白(CCSP)基因和蛋白表达的影响及量效关系。方法50只SD大鼠随机分为正常对照组、模型组、PD 1 mg·kg~(-1)治疗组、PD 1 mg·kg~(-1)预防组和PD对照组(n=10);另48只SD大鼠随机分为PD治疗组和PD预防组,各组再分别给予1、5、10和30 mg·kg~(-1)PD(n=6)。Real-time PCR法检测各组大鼠肺组织中CCSP基因表达,Western-blot法检测CCSP的蛋白表达,免疫组化法记数各组CCSP阳性细胞数。结果白藜芦醇苷显著促进CCSP在正常大鼠肺组织中的表达,还可促进内毒素休克大鼠肺组织表达CCSP,且预防组明显多于治疗组。随着PD剂量的增加,PD预防组和治疗组的CCSP的表达呈增加的趋势。结论PD可呈剂量依赖性上调内毒素休克大鼠肺组织中CCSP的基因和蛋白表达,对抗磷脂酶A2的致炎作用,实现肺保护,可能是白藜芦醇苷的重要作用机制之一。
Objective To investigate the effect of polydatin (PD) on the expression of Clara cell secretory protein (CCSP) gene and protein in lung tissue of rats with endotoxin shock and the dose-effect relationship. Methods Fifty SD rats were randomly divided into normal control group, model group, PD 1 mg·kg -1 treatment group, PD 1 mg·kg -1 prevention group and PD control group (n = 10). Another 48 SD rats were randomly divided into PD treatment group and PD prevention group. Each group was given 1, 5, 10 and 30 mg·kg -1 PD (n = 6). Real-time PCR was used to detect the expression of CCSP in lung tissue of each group. The expression of CCSP protein was detected by Western-blot. The number of CCSP positive cells in each group was recorded by immunohistochemistry. RESULTS: Polydatin significantly promoted the expression of CCSP in lung tissue of normal rats, and it also promoted the expression of CCSP in lung tissue of rats with endotoxin shock, and it was significantly more in the prevention group than in the treatment group. With the increase of PD dose, the expression of CCSP in PD prevention group and treatment group showed an increasing trend. Conclusion PD can up-regulate the expression of CCSP gene and protein in the lung tissue of rats with endotoxin shock in a dose-dependent manner. The anti-inflammatory effect of anti-phospholipase A2 and the protection of lung may be one of the important mechanisms of action of resveratrol glucoside.