【摘 要】
:
Increasing demand of artemisinin in the treatment of malaria has placed substantial stress on the total artemisinin supplies world-wide, so more attention has b
【机 构】
:
Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany,College
论文部分内容阅读
Increasing demand of artemisinin in the treatment of malaria has placed substantial stress on the total artemisinin supplies world-wide, so more attention has been paid to increasing the content of artemisinin in the Artemisia annua L. plant. In this study, amorpha-4, 11-diene synthase (ADS) cDNA (ads1) and genomics gene (gads1) were cloned from a high-yield A. annua strain 001. The activity of ADS1 was confirmed by heterogeneous overexpression of ads1 and in vitro enzymatic incubation. Reverse transcript-polymerase chain reaction results demonstrated that ads1 expressed in leaves, flowers and young stems, but not in roots. This organ-specific expression pattern of ads1 is consistent with that of artemisinin accumulation in the plant. The gads1 has a complex organization including seven exons and six introns, and belongs to class Ⅲ terpene synthase. DNA gel blotting revealed that the ADS gene has at least four copies in the genome of strain 001. The higher copy numbers might be one of the reasons for its high artemisinin content.
其他文献
为美国载人航天火箭的外层绝热瓦片的脱落问题,提出试图解决的两个方向性的四种设想方案.
介绍了一种温度补偿方法的基本原理,其解决了微型压阻式压力传感器温补问题;详细阐述了一种实用的整体补偿电路,并从理论上导出了分析计算公式,最后给出传感器整体的零位和灵
Nuclear engineering belongs to significant project; there is higher requirement on sitings. The study has discussed basic factors of selecting sites, anti-seism
概述了测区地质、岩(矿)石电性特征。根据金矿床成矿过程中有各类蚀变体—硅化体、黄铁矿化和多金属硫化物伴生,针对测区存在覆盖层厚和高压线密集的客观环境,勘查金矿时选择
With the data from the Tropical Cyclone Yearbooks between 1970 and 2001, statistical analyses were performed to study the climatic features of landfall TCs (not
随着第三代无线通讯技术的发展,手机定位已经成为一种时尚.但是利用无线通讯技术实现室内卫星定位还是一门刚刚兴起的新技术.本文就室内卫星定位技术的特点、发展和市场状况
This paper dealt with non-parallelism between the effect of microbial flocculant (MBF) on sewerage disposal and the flocculation rate, the high flocculation rat
博古昌水电站位于俄罗斯西伯利亚的安加拉河上。介绍了该水电站所处的地理位置、工程设计及施工进展情况,博古昌水电站在开发克拉斯诺雅尔斯克边疆区丰富的矿产和森林资源中
应用表达序列标签法,获得了栉孔扇贝HSP70基因cDNA的部分序列,然后利用锚定PCR技术克隆到了该基因的全长cDNA.克隆到的HSP70 cDNA全长2553bp,编码区全长1902bp,可以编码634个
There is mounting evidence that the induction of strong mucosal and cell-mediated immune responses is key element to consider in constructing efficacious HIV-1