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Be able to form large particles with protein via electrostatic force to enhance the intensity of resonance light scattering(RLS),(NH4)2SO4 was used as a RLS probe to determine bovine serum albumin(BSA),human serum albumin(HSA) and ovum albumin(OVA).The experimental conditions were investigated,including the acidity and saturation degree of (NH4)2SO4.Under the optimum conditions,the enhanced RLS intensity is proportional to the concentration of BSA,HSA and OVA in the ranges of 5×10-8―1×10-6,2.5×10-8―8×10-7 and 5×10-8―1.5×10-6 mol/L,respectively.The detection limits for BSA,HAS and OVA are 6.6×10-9,3.8×10-9 and 7.4×10-9 mol/L,respectively.The effects of foreign substances were also examined.The practical and synthetic samples were analyzed with satisfactory results.
Be able to form large particles with protein via electrostatic force to enhance the intensity of resonance light scattering (RLS), (NH4) 2SO4 was used as a RLS probe to determine bovine serum albumin (BSA), human serum albumin (HSA) and ovum albumin (OVA). The experimental conditions were investigated, including the acidity and saturation degree of (NH4) 2SO4.Under the optimum conditions, the enhanced RLS intensity is proportional to the concentration of BSA, HSA and OVA in the ranges of 5 × 10 -8-1 × 10-6, 2.5 × 10-8-8 × 10-7 and 5 × 10-8-1.5 × 10-6 mol / L, respectively. These detection limits for BSA, HAS and OVA are 6.6 × 10-9, 3.8 × 10 -9 and 7.4 × 10 -9 mol / L, respectively. These effects of foreign substances were also examined. The practical and synthetic samples were analyzed with satisfactory results.